Explore our trusted RNA extraction kits optimized for the isolation of total RNA, cell-free RNA, miRNA and mRNA from a wide range of samples

All steps for the extraction of RNA are performed at room temperature. All centrifugations are carried out using a swinging bucket rotor at 4000 x g (the maximum speed of 3500-5000 rpm …

The Qiagen RNeasy Mini Kit enables efficient RNA extraction from various tissues, but preparatory steps and sample type critically influence the success and purity of RNA.

ues on the Qiagen website: http://www.qiagen.com. The RNeasy Mini Kit from Qiagen is the kit used most. with a Quick–Start protocol for RNA extraction. You can find more detailed . …

Use an appropriate decontamination solution at the correct concentration, making sure to expose the potentially contaminated surface and/or material to the solution for the appropriate contact …

Quality control: run the RNA on an agarose gel. Store the RNA at -20oC several weeks or at -80 oC for long time.

After addition of ethanol, the sample is loaded onto an RNeasy Mini spin column. Total RNA binds to the RNeasy silica-membrane, contaminants are efficiently washed away, and high-quality …

This protocol should only be followed for use with small amounts of tissue (<30 mg); if using larger amounts of tissue follow Protocol B. The volume of lysis reagent should be at least 10 fold …

For samples stored in RNAlater do 1 cycle of 60 s). Add 100 μl chloroform (kept at 4oC), and shake vigorously for 15 s (use fume hood). Centrifuge at 14,000 x g for 20 min at 4oC. …

Oragene•RNA solution and saliva is necessary to ensure maximum RNA recovery and stability. 2. Incubate en. re sample in original vial at 50°C for 1 hour in a water bath or for 2 hou. in an air …