The Biotechnology Revolution: PCR and Cloning Expressed Genes
Jun 27, 1970 · The cloning of expressed genes and the polymerase chain reaction (PCR), two biotechnological breakthroughs of the 1970s and 1980s, continue to play significant roles in …
Cloning - Latest research and news | Nature
5 days ago · Cloning is a method that is used to produce genetically identical copies of pieces of DNA, cells or organisms. Cloning methods include: molecular cloning, which makes copies of …
An optimized disease resistance gene cloning workflow for wheat
May 28, 2025 · Gene cloning in wheat has long been hampered by its exceptionally large and complex genome, and long life cycle. Here, the authors report an optimized, high-throughput …
recombinant DNA technology / DNA cloning; gene cloning; …
recombinant DNA technology / DNA cloning; gene cloning; cloning A technology that uses enzymes to cut and paste together DNA sequences of interest.
Golden EGG, a simplified Golden Gate cloning system to ... - Nature
Oct 25, 2024 · The simplified Golden Gate cloning system. The pEGG entry vectors contain a cassette with the ccdB and cat genes for negative selection that is flanked by outward directed …
Efficient cloning of linear DNA inserts (ECOLI) into plasmids using ...
Sep 16, 2024 · This study introduces a novel cost-effective technique for cloning of linear DNA plasmid inserts, aiming to address the associated expenses linked with popular in vitro DNA …
A multi-kingdom genetic barcoding system for precise clone
May 21, 2025 · A barcoded CRISPR base editing system isolates target clones from complex mammalian, yeast and bacterial populations.
DNA cloning by homologous recombination in - Nature
The cloning of foreign DNA in Escherichia coli episomes is a cornerstone of molecular biology. The pioneering work in the early 1970s, using DNA ligases to paste DNA into episomal …
Mammalian heparanase: Gene cloning, expression and function in …
Jul 1, 1999 · Cloning of the heparanase gene enables the development of specific molecular probes for early detection and treatment of cancer metastasis and autoimmune disorders.
Rapid cloning of disease-resistance genes in plants using ... - Nature
Apr 25, 2016 · A method for rapid cloning of plant disease-resistance genes could provide sustainable, genetic solutions to crop pests and pathogens in place of agrichemicals.